Course Overview

Want to gain a fundamental understanding of growth of mammalian cells in bioreactors, and production of recombinant proteins?

Then sign up for this course where you will also learn about cell culture media, cell banking, scale-up and down, and single-use bioreactor versus stainless steel bioreactor design in cell culture development. Get hands-on experience with mammalian bioreactors, from 15 mL to 50L, and a variety of single-use biomanufacturing options e.g. rocker bags, stirred tank and perfusion.

In addition to extensive hands-on experience with the process, this course will also address many of the common concerns when implementing single-use processes – i.e., sterile connections, environmental concerns/carbon footprint, business economics, and validation.

Learning Outcomes

At the end of this course, participants will be able to:  

• Design of animal cell culture bioreactors, including suspension and immobilized cell bioreactors, conventional stainless steel stirred tanks, and disposable (single-use) bioreactors
• Identify advantages and disadvantages of single-use technologies relative to multi-use equipment
• Understand the pros and cons for bioreactor types (rocker, stirred tank, microcarrier) and strategies (batch, fed-batch, perfusion)
• Setup and operation of single-use options for upstream manufacturing (rocker, stirred tank and perfusion)
• Advanced automation and control of animal cell culture processes, including bioreactor PAT
• Basic cost calculations to compare single-use options to more traditional multi-use set ups, to provide justification for single-use technologies.

Who should attend?

This course is intended for professionals within the biopharmaceutical industry who want to:

• Gain hands-on experience with mammalian bioprocessing equipment
• Gain an understanding of upstream mammalian bioprocessing
• Work indirectly with mammalian bioprocessing and would like to better understand it

Prerequisites 

This course has no specific prerequisites. It is an introductory course with a focus on hands-on training.